The effect of basic fibroblast growth factor on the mRNA expression of beta1 integrin subunit by periodontal ligament fibroblasts in culture / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the effect of basic fibroblast growth factor on the mRNA expression of beta1 integrin subunit by periodontal ligament fibroblasts in culture; to discuss the effect of basic fibroblast growth factor in periodontal regeneration.</p><p><b>METHODS</b>Human periodontal ligament fibroblasts were cultured and stimulated by basic fibroblast growth factor (0.1, 1.0, 10.0 ng x mL(-1)) for 24, 48, 72 h respectively, and then mRNA expression of beta1 integrin subunit was assessed by fluorescent quantitative real-time reverse transcription polymerase chain reaction.</p><p><b>RESULTS</b>Basic fibroblast growth factor enhanced the mRNA expression of beta1 integrin subunit, and there was optimal effect when the concentration of basic fibroblast growth factor was 1.0 ng x mL(-1) at 24, 48, 72 h respectively; the mRNA expression of beta1 integrin subunit at 72 h was higher than that at 24, 48 h.</p><p><b>CONCLUSION</b>Basic fibroblast growth factor can strengthen human periodontal ligament fibroblasts' adhesion and may be one of important factors which participate in the periodontal regeneration.</p>

Effect of basic fibroblast growth factor on the gene expression of epidermal growth factor receptor by periodontal ligament cells in culture / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the effect of basic fibroblast growth factor (bFGF) on the gene expression of epidermal growth factor receptor (EGFR) by periodontal ligament cells (PDLC) in culture and discuss the effect of bFGF in cell differentiation and periodontal regeneration.</p><p><b>METHODS</b>Human PDLC were cultured in vitro and cell clone was obtained by the method of limiting dilution. PDLC were stimulated by bFGF, and then gene expression of EGFR was assessed by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The mRNA expression of EGFR was promoted by bFGF and the effect was dose-dependent.</p><p><b>CONCLUSION</b>The promotion effect of the mRNA expression of EGFR in PDLC may be one of important factors which participate in the healing process of periodontitis and provide partly theoretical basis of cell differentiation and periodontal regeneration.</p>

Heterotopic complex membrane induces bone formation / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The composite membrane was made by recombinant human bone morphogenetic protein-2 (rhBMP-2), collagen (Co), polylactic-co-glycolic acid (PLGA). To observe the ectopic bone formation and evaluate the capability of rhBMP-2/Co/PLGA complex membrane.</p><p><b>METHODS</b>48 male Kunming adult mice were divided into two groups randomly: Co/PLGA complex membrane group and rhBMP-2/Co/PLGA complex membrane group. After etherized, an incision was made on the outer muscle and two groups of complex membranes were implanted into the right side hind limb muscle pouch. 8 mice from each group were sacrificed at 7, 14, 28 d. The hind limbs were removed, examined by soft X-ray, HE staining and light microscope examination.</p><p><b>RESULTS</b>Wounds healed well, no infections and rejections were observed. In the Co/PLGA group, partial implanted membrane degradated and absorbed at 7 d. The membrane collagen fiber appeared loose at 14 d. The membrane lost its intact to disrupted at 28 d. No ectopic bone formation was found. In the rhBMP-2/Co/PLGA group, ectopic bone formation was found by eye, soft X-ray and histological examinations. The rhBMP-2/Co/PLGA complex membrane degraded slower than Co/PLGA complex membrane.</p><p><b>CONCLUSION</b>Co/PLGA can provide a carrier for rhBMP-2 to have stronger effect of biological activity.</p>

The effect of basic fibroblast growth factor on the gene expression of decorin by periodontal ligament fibroblasts in culture / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the effect of basic fibroblast growth factor (bFGF) on the gene expression of decorin by periodontal ligament fibroblasts (PLFs) in culture, and discuss the effect of bFGF in periodontal regeneration.</p><p><b>METHODS</b>Human PLFs were cultured and stimulated by exogenous bFGF. Gene expression of decorin was assessed by semi-quantitive RT-PCR.</p><p><b>RESULTS</b>The mRNA expression of decorin was suppressed by bFGF and the effect was dose-dependent. When the dose of bFGF increased, the inhibitive effect decreased.</p><p><b>CONCLUSION</b>Decorin has many biological effects. The inhibitive effect may be one of important factors which participate in the healing process of periodontitis, and provide partly theoretical basis of bFGF in periodontal regeneration.</p>

Papillon-Lefevre syndrome: a case report / 华西口腔医学杂志

Papillon-Lefevre syndrome (PLS) is an extremely rare inherited disease as an autosomal recessive trait. The disorder is characterized by diffuse palmoplantar keratoderma and premature loss of both deciduous and permanent teeth. This paper described a case of PLS with classic clinical features and briefly reviewed the relevant literature.